Within the research project ZF-Health (Zebrafish Regulomics for Human Health), ZF BioLabs has generated two morphant zebrafish for two rare diseases associated withmitochondrial enzymes, Friedreich’s ataxia and Leigh Syndrome, and has performed a moderate-throughput screening of compounds to identify suppressors of disease phenotypes. http://www.zf-health.org/.
The project is being developed by a Pan-European research consortium formed by 19 different organizations and ZF Biolabs is the only Spanish organization within the consortium.
ZF BioLabs collaborated as a partner in an European Research Project on laminophaties, using the Zebrafish as an animal model to study in vivo the molecular mechanisms of the disease pathology.
New screening R&D searching for anti-ageing compounds based on zebrafish embryos. We developed a method to induce senescence associated to oxidative stress in zebrafish embryos.
We studied the senescent level by measuring the biochemical marker SA-beta-galactosidose. We also performed analysis of genetic markers related to ageing processes. Funded by Comunidad de Madrid
ALTERNATIVE TESTING METHODS
ZF BioLabs was the Coordinator of an European project that lead to an European Network for alternative testing strategies in ecotoxicology; EUROECOTOX under the Seven Framework Program (FP7).
R&D for Biological and Technological Advancements in Ecotoxicology
Together with a large water supply company (Canal de Isabel II) ZF BioLabs developed the technology Fast acute Toxicity Test with ZebrafIsh that is very useful to detect toxicity of different compounds in a very short period of time. Funded by Comunidad de Madrid
Development of Acute Toxicity Test
During this R&D project, ZF Biolabs developed a new alternative method based on Zebrafish embryos to evaluate acute toxicity and toxicogenomics of new drugs. Funded by Comunidad de Madrid
Evaluating in vivo toxicity of nanomaterials
It is important for nanomaterials R&D projects to include toxicological and safety evaluation so that actual risks of nanomaterials are defined and adverse environmental consequences are minimized. In collaboration with the Institute of Materials Science of Aragon (ICMA), ZF Biolabs has participated in the evaluation of different nanomaterials.
New alternative method to evaluate cardiotoxicity of compounds
ZF Biolabs developed the ZF S-Cardiac kit to identify potential cardiotoxic effects of drugs based in the Zebrafish embryo model. Funded by Comunidad de Madrid.
Determination of bioconcentration in ecotoxicity bioassay included in the REACH normative
This project was a Cooperation Project with the Analytical Chemical Department of Complutense University of Madrid and the Instrumental Analysis and Environmental Chemistry Department (CSIC’s Institute of General Organic Chemistry). The main aim of the project was to develop a method for determination of the bioconcentration factor using Zebrafish embryos that serves as an alternative to the adult fish bioassay 305 of OECD. Funded by the agriculture, food and environment ministry.
R&D for a novel developmental toxicity assay
During this R&D project ZF BioLabs created a developmental toxicity test using Zebrafish embryos as an alternative to the mammalian model and with the very deep knowledge of the Zebrafish embryology adquired, an Atlas of Malformations was compiled. Funded by Comunidad de Madrid.
R&D of new natural products with photo-protective properties
In this project ZF BioLabs worked as CRO with a large Spanish Pharmaceutical company (Industrial Farmaceutica Cantabria S.A.) testing new cosmetic compounds against the damage caused by ultraviolet radiation. It was developed a screening system for zebrafish embryos to detect the photoprotective activity of natural extracts from seaweed.
New vertebrate model for ageing and Systems Biology
ZF Biolabs developed a new vertebrate model for ageing and Systems Biology approach, a mathematical model of relevant age-dependant biological processes (growth, reproduction and mortality) in zebrafish. This knowledge could be applied to develop a Quantitative System Toxicology (QST) platform, taking advantage of zebrafish as a toxicology animal model.
The work was presented at the “International Symposium on “Developmental origins of health and disease” held in Greifswald (Germany) on September 4th and 5th 2009, organized by the German National Academy of Sciences “Leopoldina”.