We perform the evaluation of the acute toxicity LC50 of the selected compounds and we test four morphological endpoints of acute lethality in 48 hours.
FAST ACUTE TOXICITY ASSAY
We have also developed a Fast Acute toxicity bioassay using zebrafish embryos just after fertilization, which produces reliable data with only 1,5 hours exposure to the test compound.
TERATOGENICITY / DEVELOPMENTAL TOXICITY ASSAY
We use definitive endpoints to establish the teratogenicity of a specific compound, based on a very deep knowledge of the embryology of the Zebrafish. The ZF Biolabs developmental toxicity assay is a valid alternative for texting embryotoxicity.
Correlation of results with mammalian modes is well established. A teratogenic index of each compound is determined based on LC50 and EC50 results at 48 hours post fertilization. Data are available after 48 hours.
ORGAN-SPECIFIC TOXICITY ASSAYS
The purpose of this assays are to determine whether toxicity is focused on any organ. In zebrafish, the heart beat starts around 24 hours after fertilization and other organs (liver, gut, kidneys and blood vessels) are formed in the first 48 hours of development.
Taking into account the transparency of the embryos, organ-specific toxicity can be monitored directly (cardiotoxicity, for example) or using antibodies or dyes targeted to the different organs (neurotoxicity, nephrotoxicity or hepatotoxicity, for example, can be monitored by this means).
The Zebrafish embryo model is an effective and accurate tool for raplidly assesses nanomaterial toxicity at low cost. This model is also well suited to identify areas of relatively distribution of nanomaterials that are fluorescently labeled. ZF Biolabs offers a nanomaterial toxicity bioassay including LC50 and EC50 of several morphological and functional endpoints. Information gained from this dynamic in vivo assay can be applied to predict effects in other systems since genetic, cellular and physiologiacal processes are highly conserved between zebrafish and human, especially early in development.
For ecotoxicology studies, two OCDE test guidelines have been already published OECD 236 (Fish Embryo Acute Toxicity o FET) y OECD 212 (Fish, Short-term Toxicity Test on Embryos and Sac-fry Stages), being zebrafish the recommended species.