Why use Zebrafish Embryos?
- It is a whole vertebrate organism but small enough to be handled in multiwell plates.
- Provides more and better information than cell models.
- Represent good correlation to mammal models: 80% homology with human genome.
- Small and optically transparent body suitable for real-time microscopic observations.
- Requires small amount of testing compound compared to other vertebrate models.
- Zebrafish embryos have the ability to absorb compounds directly from water. In addition, testing compounds with very low solubility could be assessed by microinjection.
- Faster and less costly than rodents.
- It is not considered laboratory animal by EU Directive (EU Dir 86/609/CEE regarding protection of animals used for experimental research and other scientific objectives).
- Complies with the principle of the 3Rs: Reduce, Refine & Replace.
- Make statistics in one round!!: Statistically significant numbers of specimens are used per test.
Where Zebrafish can be Useful?
- In vivo pharmacology/Safety Pharmacology
- Toxicity testing
- General toxicology: acute and chronic
- Ecotoxicology: effects on population, community and ecosystems of toxic chemicals
- Nanotoxicology: toxicity studies on nanomaterials.
- Reproductive Toxicity (Teratogenicity).
- Development of a quantitative system toxicology (QST) platform: Systems Toxicology combines conventional toxicological assessment strategies with system-wide measurement methods (analysis of large networks of molecular and functional changes occurring across multiple levels of biological organization) and computational analysis The zebrafish can be a very useful tool to obtain huge amounts of experimental data (High Throughput Screening) in a very cost-effective way.
- Application of Zebrafish embryos in cosmetics.