Why Zebrafish?

Why use Zebrafish Embryos?

  • It is a whole vertebrate organism but small enough to be handled in multiwell plates.
  • Provides more and better information than cell models.
  • Represent good correlation to mammal models: 80% homology with human genome.
  • Small and optically transparent body suitable for real-time microscopic observations.
  • Requires small amount of testing compound compared to other vertebrate models.
  • Zebrafish embryos have the ability to absorb compounds directly from water. In addition, testing compounds with very low solubility could be assessed by microinjection.
  • Faster and less costly than rodents.
  • It is not considered laboratory animal by EU Directive (EU Dir 86/609/CEE regarding protection of animals used for experimental research and other scientific objectives).
  • Complies with the principle of the 3Rs: Reduce, Refine & Replace.
  • Make statistics in one round!!: Statistically significant numbers of specimens are used per test.

Why zebrafish

Where Zebrafish can be Useful?

  • In vivo pharmacology/Safety Pharmacology
  • Toxicity testing
    • General toxicology: acute and chronic
    • Ecotoxicology: effects on population, community and ecosystems of toxic chemicals
    • Nanotoxicology: toxicity studies on nanomaterials.
  • Reproductive Toxicity (Teratogenicity).
  • Development of a quantitative system toxicology (QST) platform: Systems Toxicology combines conventional toxicological assessment strategies with system-wide measurement methods (analysis of large networks of molecular and functional changes occurring across multiple levels of biological organization) and computational analysis The zebrafish can be a very useful tool to obtain huge amounts of experimental data (High Throughput Screening) in a very cost-effective way.
  • Application of Zebrafish embryos in cosmetics.